Arumemi, Fortuna and Bayles, Ian and Paul, Joshua and Milcarek, Christine (2013) Shared and discrete interacting partners of ELL1 and ELL2 by yeast two-hybrid assay. Advances in Bioscience and Biotechnology, 04 (07). pp. 774-780. ISSN 2156-8456
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Abstract
ELL2 (eleven-nineteen lysine-rich leukemia transcription elongation factor), a component of a larger complex with pTEFb (cyclin T and CDK9) and AF4, is up-regulated in plasma cells where it influences mRNA processing by increasing exon skipping and enhancing proximal poly (A) site use. ELL2 is needed to produce the secretory-specific Ig heavy chain mRNA while ELL1 mRNA does not change in abundance with B cell stages. To investigate the potential interactions of other proteins with the ELL1 and ELL2 proteins, we preformed yeast two-hybrid studies. HSP40 and Testin were found to bind to ELL2 in its amino-terminal half. PCNA binds to ELL2 in a region encompassing amino acids 186 - 344. The potent transcription factors HIF1 α and ZNF622 interact with both ELL1 and 2 in the central, proline rich region. Meanwhile, BBS2 and ING3 interact with ELL1 but not ELL2 in this central proline-rich region. Many of the ELL-interacting-proteins uncovered in the two-hybrid screen are tumour suppressors that may work through the ELL: pTEFb complex to suppress or activate sets of genes in plasma cells.
Item Type: | Article |
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Subjects: | Open STM Article > Biological Science |
Depositing User: | Unnamed user with email support@openstmarticle.com |
Date Deposited: | 23 Mar 2023 07:02 |
Last Modified: | 27 Apr 2024 11:01 |
URI: | http://asian.openbookpublished.com/id/eprint/341 |