Fajobi, A. O. and Okon, B. O. Emma- and Oyedapo, O. O. and Dare, C. A. and Akinlalu, A. O. and Komolafe, I. J. and Ogunsusi, M. (2020) Cardioprotective Activities of Pterocarpus mildbraedii Leaves on Isoproterenol-induced Myocardial Infarction in Rats. European Journal of Medicinal Plants, 31 (10). pp. 20-37. ISSN 2231-0894
![[thumbnail of 909-Article Text-1824-1-10-20221012.pdf]](http://asian.openbookpublished.com/style/images/fileicons/text.png)
909-Article Text-1824-1-10-20221012.pdf - Published Version
Download (2MB)
Abstract
The study evaluated the effects of Pterocarpus mildbraedii leaf extracts on myocardial infarction induced by isoproterenol (ISO) in Wistar albino rats with a view to ascertain the value of its use in the management of heart-related diseases. Fresh plant leaves were collected, identified, extracted, fractionated and the aqueous layer partitioned with ethyl acetate. GC-MS was carried out on the ethyl acetate fraction (EAF) and unknown compounds were identified by comparing measured mass spectral data with those in NIST 14 Mass Spectral Library. Twenty-five adult rats were divided into five groups of 5 rats each. Groups I &II were the control groups. Rats in groups III-V were pretreated with 50 mg/kg and 100 mg/kg of EAF and 1.8 mg/kg of propranolol respectively for 21 days. Myocardial infarction was then induced in all the rats (except those in Group I) with the intraperitoneal injection of ISO (85 mg/kg) for 2 days. Afterwards, the rats were sacrificed and blood samples, heart homogenate and samples for histological studies were aseptically collected. Activities of cardiac biomarkers, lipid profile, enzymatic and non-enzymatic antioxidants were evaluated using standard methods. GC-MS analysis showed that the most abundant components of the plant are propionic acid, 2,3-dimethylphenyl ester, catechol, octyl-β-D-glucopyranoside, phenol and n-Hexadecanoic acid. Administration of ISO caused significant elevation of the activities of cardiac biomarkers (troponin-T concentrations, creatine kinase-MB and lactate dehydrogenase) while rats pretreated with EAF had significantly lower levels of the biomarkers. Moreover, alterations in lipid profile, enzymatic and non-enzymatic antioxidants brought about by the administration of ISO were ameliorated. Histological examinations revealed lesser degree of myocardial injury in pre-treated rats.
Item Type: | Article |
---|---|
Subjects: | Open STM Article > Medical Science |
Depositing User: | Unnamed user with email support@openstmarticle.com |
Date Deposited: | 13 Apr 2023 06:59 |
Last Modified: | 28 May 2024 05:35 |
URI: | http://asian.openbookpublished.com/id/eprint/254 |