Synthesis and Characterization of Copper Oxide Nanoparticles from Aqueous Extract of Cyanotis tuberosa (Roxb.) Schult. & Schult. F. and Analysis of Antioxidant, Antimicrobial and Anticancer Activity

Cyanotis tuberosa (Roxb.) Schult. & Schult.f family Commelinaceae is traditionally used as ethnomedicine for curing several health problems in India. In this study copper oxide CuONPs were synthesized using C. tuberosa tubers aqueous extract. The biosynthesized CuONPs were characterized using an Ultra violet–visible spectroscopy, Fourier-Transform Infra Red spectroscopy, X-RAY Diffraction Analysis, Scanning electron microscopy. The results obtained from SEM showed that the copper oxide nanoparticles were a face-centered cubic crystal structure. The characterization of the biosynthesized CuONPs through EDS also indicated that the reaction product was composed of highly pure CuO NPs. The invitro antimicrobial activity of different concentrations of green synthesized copper oxide nanoparticles of C. tuberosa showed that the highest inhibition zone was observed on Klebsiella pneumonia (15.25 mm). Furthermore, the antioxidant activity of green synthesized CuONPs of C. tuberosa tubers aqueous extract was also tested the highest percentage activity was exhibited at 100 μg/ml CuONPs (56.32). The human cancer cell line was used for cytotoxicity analysis at 48 hrs of incubation period, a significant abatement in cell viability was observed against the treated cell lines. The study concludes that the C. tuberosa tubers aqueous extract can be successfully used for the synthesis of CuONPs that exhibit effective in vitro anti-bacterial, antioxidant and anticancer activity.